Histomorphometric evaluation of bone healing in rabbit fibular osteotomy model without fixation

نویسندگان

  • Marcos A Matos
  • Francisco P Araújo
  • Fabio B Paixão
چکیده

BACKGROUND Animal models of fracture consolidation are fundamental for the understanding of the biological process of bone repair in humans, but histological studies are rare and provide only qualitative results. The objective of this article is to present the histomorphometric study of the bone healing process using an experimental model of osteotomy in rabbit fibula without interference of synthesis material. METHODS Fifteen rabbits were submitted to fibular osteotomy without any fixation device. Groups of five animals were submitted to pharmacological euthanasia during a period of one (group A), two (group B) and four weeks (group C) after osteotomy. Histomorphometric evaluation was performed in the histological sections. RESULTS During week one there was intense cellularity (67/field), a large amount of woven bone (75.7%) and a small amount of lamellar bone (7.65%). At two weeks there was a decrease in woven bone (41.59%) and an increase in lamellar bone (15.16%). At four weeks there was a decrease of cellularity (19.17/field) and lamellar bone (55.56%) exceeded the quantity of woven bone (31.68%). CONCLUSION Histomorphometric (quantitative) evaluation of the present study was shown to be compatible with bone healing achieved in qualitative experimental models that have been commended in the literature.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Systemic Effects of Experimental Spinal Cord Injury on Bone Healing in Rabbit

Bone loss after spinal cord injury leads to increased fragility of bone and subsequent risk for low-trauma fractures in the sublesional parts of the body. Although in such injuries upper limbs are normally innervated, bone loss may occur in the upper extremities. The present study was designed to determine the systemic effects of spinal cord injury on the fracture healing of upper limbs in rabb...

متن کامل

Evaluation of the effects of autologous adipose derived mesenchymal stem cells in combination with polyacrylamide hydrogel and nanohydroxyapatite scaffolds on healing in rabbit critical-sized radial bone defect model

Objective: In this study, the bone regeneration ability of polyacrylamide hydrogel and nanohydroxyapatite scaffolds (PAAH/NHA) and stem cells derived from adipose tissue (ADSCs) in the healing of critical sized bone defects in rabbit radius were assessed. Animals and procedures: 12 New Zealand white male rabbits were divided into 3 groups. The rabbits were anesthetized and 15 mm bone def...

متن کامل

Leptin-deficiency eradicates the positive effect of traumatic brain injury on bone healing: histological analyses in a combined trauma mouse model

INTRODUCTION The combination of traumatic brain injury (TBI) and long-bone fracture leads to increased formation of callus and mineral density in wild-type (WT) mice. However, this effect was not detected radiologically in leptin-deficient mice. Due to the complex interactions between hormonal and bone metabolism and the important role of leptin in this setting, our aim was to investigate morph...

متن کامل

A rabbit humerus model of plating and nailing osteosynthesis with and without Staphylococcus aureus osteomyelitis.

The local mechanical environment at a fracture is known to influence biological factors such as callus formation, immune cell recruitment and susceptibility to infection. Infection models incorporating a fracture are therefore required to evaluate prevention and treatment of infection after osteosynthesis. The aim of this study was to create humane, standardised and repeatable preclinical model...

متن کامل

Effect of a static magnetic field on bone healing in the dog: radiographic and histopathological studies

Although the promotional effects on bone healing of pulsed electromagnetic fields (PEMF) have beenwell demonstrated, the effects of static magnetic fields (SMF) remained unclear. In this study, effects of acustom-made magnetic wrap on radiographic and histopathological aspects of bone healing using a canineunstable osteotomy gap model were investigated. After an osteotomy of the midshaft radius...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of Orthopaedic Surgery and Research

دوره 3  شماره 

صفحات  -

تاریخ انتشار 2008